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KMID : 1094020160330050266
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Journal of Veterinary Clinics
2016 Volume.33 No. 5 p.266 ~ p.269
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The Potential and Accuracy of RNA-based Fetal Sex Determination during Early Pregnancy Using Cell-Free Fetal RNA from Korean Native Cows (Bos taurus coreanae)
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Lee Sang-Ho
Oh Ki-Seok
Park Chul-Ho
Kim Yong-Min
Lee Jin-A
Sohn Seong-Won
Son Chang-Ho
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Abstract
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Cell-free fetal RNA is useful to determine fetal sex and detect other inherent genetic disorders. However,non-invasive fetal sex determination methods using fetal RNA from maternal plasma is not yet well established instudies pertaining to bovine animals. Thus, the aim of this study was to systematically evaluate the presence of themale-specific ZRSR2Y gene transcript in maternal plasma using Reverse Transcriptase-Polymerase Chain Reaction (RTPCR)assays, and to verify its accuracy, sensitivity, and specificity in determining fetal sex between 30 and 100 daysof gestation. Overall accuracy, sensitivity, and specificity of the ZRSR2Y gene transcripts in determining fetal sexwere 89.1%, 86.3%, and 100%, respectively. The 30 to 100 days of gestation were further classified into five stagesof gestation, and each stage had relatively high accurate, sensitive, and specific results. Overall, these results indicatethat the expression of the ZRSR2Y gene can be used for fetal sex determination in bovine animals using circulatingcell-free RNA in maternal plasma during early pregnancy.
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KEYWORD
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cell-free fetal RNA, early pregnancy, fetal sex determination, bovine
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